Most Yeast Plasmid Transformations Performed In A Single Sitting

United States Michael Smith

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Princeton, New Jersey, United States / April 14, 2010

Michael Smith of Princeton University’s Rose Lab successfully performed 147 yeast plasmid transformations by hand in a single sitting. Each transformation was conducted in a microcentrifuge tube, and then plated onto -URA glucose media.

- each transformation must be successful, including sterile            controls 
- attempter need not wait for yeast to grow, although each transformation must be plated to prove successful transformation

Comments

  • Michael, this is fantastic! You can come work in the Brodsky lab any day!

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