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147 transformations

Most Yeast Plasmid Transformations Performed In A Single Sitting

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Tags: most, Science and Technology, laboratory, scientific, yeast

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response to this attempt

Mon, 14 Jun 2010 13:18:36 GMT

Michael, this is fantastic! You can come work in the Brodsky lab any day!

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Set By

Michael Smith Princeton, New Jersey

April 14, 2010

INFO

Michael Smith of Princeton University’s Rose Lab successfully performed 147 yeast plasmid transformations by hand in a single sitting. Each transformation was conducted in a microcentrifuge tube, and then plated onto -URA glucose media.

Rules

- each transformation must be successful, including sterile controls
- attempter need not wait for yeast to grow, although each transformation must be plated to prove successful transformation

Current 147 transformations Set by Michael Smith

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