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19 frog plates

Most Handmade Yeast Frogging Plates Prepared In A Single Sitting

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Tags: most, Science and Technology, yeast, laboratory, petri dish, galactose, frog plate

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Mon, 01 Jan 0001 00:00:00 GMT

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Thu, 15 Apr 2010 22:00:52 GMT

Should I be worried?
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Thu, 15 Apr 2010 22:00:39 GMT

Should I be worried?
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Rob Birdsong
Wed, 14 Apr 2010 10:21:47 GMT

Fixed Thanks Miguel
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Michael Smith
Tue, 13 Apr 2010 19:32:18 GMT

That's Professor Rose. Not Professor Dunbar.

Set By

Michael Smith

Set On

March 2, 2010

Location

Princeton, New Jersey

Info

Michael Smith prepared 19 frog plates by hand in a single sitting. He performed the feat in the Rose Lab at Princeton University.

Each frog plate is a 96-well plate with strains of yeast at the top, diluted down the lanes. Each frog plate was plated onto at least two plates to confirm growth (-URA and -URA Galactose). Professor Mark Rose was present to witness the achievement.

See full-sized images here and here.

Rules

- each frog plate must be prepared on a 96 well plate
- the 'frog area' is defined as an 8x6 matrix
- each frog plate must be plated to at least two growth plates to prove yeast present at highest dilution.
- the whole process must be done in a single sitting, from inoculation, to serial dilutions, to growth plating,
- participant need not be present during yeast growth